DNA Manipulation Questions

Back to Topic Selection Unit 3 Q&A: Plasma Membrane Q&A: Nucleic Acids and Proteins Q&A: Genes and Regulation Q&A: Biochemical Pathways Q&A: Photosynthesis Q&A: Cellular Respiration Q&A: Cell Signalling Q&A: Responding to Antigens Q&A: Immunity Unit 4 Q&A: Mutations Q&A: Genetic Makeup Q&A: Biodiversity Overtime Q&A: Relatedness Q&A: Human Evolution Q&A: DNA Manipulation Q&A: Biotechnology and Society Q&A: Biotechnology and Disease

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Q1. What property does DNA have that enables it to be transferred from one species to another?

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Q2. Suggest why the use of restriction enzymes and gel electrophoresis may not be possible with a mutation

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Q3. Bacterial plasmids can be used in the cloning of foreign genes. Explain why plasmids can be used for this purpose.

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Q4. A DNA polymerase enzyme is involved in the Polymerase Chain Reaction (PCR) process. Explain the role of the polymerase enzyme in PCR.

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Q5. Describe a characteristic of gel electrophoresis

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Q6. Explain why the DNA polymerase enzyme used is heat resistant

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Q7. Explain why the same restriction enzyme must be used to cut the fragments in preparing recombinant plasmids

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Q8. Describe the technique of gel electrophoresis and give a use of this technique

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Q9. Outline the method known as PCR that is used to amplify DNA

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