Introduction

Enzymes are large globular proteins that reduce the activation energy required to initiate a reaction as they weaken the bonds in the substrate. Therefore, they act as organic catalysts by increasing the rate of chemical reactions. Their function primarily depends on the presence of an active site or substrate binding site, which is a region that has a complementary 3D shape to the substrate.

Key Components

Before we begin, here are some terms which you should familiarise yourself with.

  • Activation Energy: the energy required to initiate a reaction
  • Catalysts: substances which accelerate a reaction without being consumed
  • Substrate: the substance(s) which an enzyme acts upon

Types of Chemical Reactions

  • Anabolic and Endergonic: energy is absorbed by combining smaller molecules
  • Catabolic and Exergonic: energy is released by splitting up large molecules

Models of Enzyme Function

There are currently two models for how enzymes function, the lock and key model and the induced fit model which is more recent and plausible. The lock and key model states that the active site is an exact match for its substrate. While the induced fit model states that the active site is a close match for the substrate, but the binding of the substrate induces a conformation change allowing it to bind.



Induced Fit Model

Factors Affecting Enzymatic Activity

The functioning of enzymes relies heavily on the 3D shape of their active site. Any factor that alters this will affect the activity of the enzyme as it may inhibit the formation of substrate-enzyme complexes.

Temperature

All enzymes have optimal temperatures where they operate most efficiently. For Example, most humans enzymes have an optimum temperature of around 37°C, the normal body temperature. When the temperature increases, the molecules would have more kinetic energy, resulting in more collisions between the substrates and enzymes. However, if the temperature is increased beyond the optimum temperature, this causes the enzyme to denature, and hence the rate of the reaction to rapidly decrease. When an enzyme denatures, the bonds within the enzyme break and the tertiary structure unravels, leading to the loss of its conformation 3D shape, and therefore the substrate can no longer bind with the active site. When the temperature is decreased, there is less kinetic energy available and the movement between the enzymes and substrates will decrease, thereby slowing the reaction. Ultimately, freezing the reactants will stop the reactions, however it can be reversed.

pH

Just like temperature, all enzymes have an optimal pH level. If the pH deviates significantly either side of its optimum value, then this will cause changes within the hydrogen bonding within the enzyme changing the 3D structure and thus inhibiting its activity.

Inhibitors

There are two types of inhibitors, competitive inhibitors and non-competitive inhibitors. If the inhibitor covalently binds to the enzyme, permanently altering the structure of the enzyme then it is referred to as being irreversible inhibition.

Competitive inhibitors work by having a similar shape to the usual substrate molecule and so they can bind to the active site of the enzyme, preventing the formation of enzyme-substrate complexes. These enzymes can be overcome by increasing the concentration of the substrate, as this increases the likelihood that a substrate molecule will interact with the active site instead of an inhibitor molecule.

Non-competitive inhibitors work by binding to an allosteric site on the enzyme. By binding to the allosteric site, it causes a conformational 3D change within the enzyme, changing the shape of the active site and thereby preventing it from binding to its substrate.

Typically, competitive inhibitors are reversible, while non-competitive inhibitors are irreversible.



Affect of Increasing Temperature



Affect of Inhibitors

Cofactors

Many enzymes require other, non-protein molecules to be present to activate or increase the rate of an enzyme. Coenzymes are a subset of cofactors and we will discuss some specific examples later when we cover photosynthesis and cellular respiration. Some enzymes also require inorganic ions, metal ions such as Mg2+ and Cu2+